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Detection of activated caspase-8 in living cells. (100 assays)
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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: Differential Regulation of NOTCH2 and NOTCH3 Contribute to Their Unique Functions in Vascular Smooth Muscle Cells
doi: 10.1074/jbc.m115.655548
Figure Lengend Snippet: FIGURE 5. NOTCH3 protects smooth muscle cells from cell death. HAoSMCs with lentivirally overexpressed NICD2, NICD3, and GFP, or siRNA knockdown of NOTCH2 (siN2), NOTCH3 (siN3), and control (siCTL) were exposed to 50 J/cm2 UV radiation and collected for protein 9 h later. A and B, Western blots to detect NOTCH2 and NOTCH3 expression, and level of total and cleaved caspase3 with tubulin used as loading control, n 3. C and D, caspase3 enzymatic activity assay using fluorescent caspase substrate AFC-DEVD, n 4. Significance determined by one-way ANOVA; *, p 0.05.
Article Snippet: Lysates were then used in a
Techniques: Knockdown, Control, Western Blot, Expressing, Enzyme Activity Assay
Journal: Journal of Biological Chemistry
Article Title: Differential Regulation of NOTCH2 and NOTCH3 Contribute to Their Unique Functions in Vascular Smooth Muscle Cells
doi: 10.1074/jbc.m115.655548
Figure Lengend Snippet: FIGURE 7. Genetic deletion of Notch3 has unique effects on the survival of mouse aortic smooth muscle cells. A, mRNA expression of pro-survival genes in aortas of wild-type (WT), Notch2-deficient (Notch2fl/fl; MCC/), and Notch3-mutant (Notch3/) adult mice. qPCR with relative expression compared with GAPDH, n 4. B, SMCs were isolated and cultured from aortas of wild-type, Notch2, and Notch3-deficient mice and used in enzymatic caspase3 activity assays, n 4. C, total and phosphorylated (phospho)-ERK was measured in cultured smooth muscle cells following serum challenge, with tubulin used as loading control, n 4. Significance determined by one-way ANOVA, *, p 0.05.
Article Snippet: Lysates were then used in a
Techniques: Expressing, Mutagenesis, Isolation, Cell Culture, Activity Assay, Control